Robuta

https://docs.google.com/document/d/16g22SmTFp7whqmNeCFUsvVkWG-WYzZ-O/edit?usp=sharing&ouid=110326099826710233402&rtpof=true&sd=true Nunez lab sgRNA cloning protocol BstXI BlpI.docx - Google Docs Cloning sgRNA sequences for S. pyogenes (d)Cas9 *This protocol uses the Weissman/Gilbert lab vector for expressing sgRNAs (pLG1 backbone) pLG1:... nunezlabsgrnacloningprotocol