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Author Summary Ebola virus (EBOV) causes severe hemorrhagic fever with case fatality rates of up to 90% and no therapy or vaccine currently available. 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A novel interaction of tRNA(Lys,3) with the feline immunodeficiency virus RNA genome governs initiation of minus strand DNA synthesis. https://www.mdpi.com/1999-4915/13/4/668 Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology RNA viruses cause a wide range of human diseases that are associated with high mortality and morbidity. 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